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Applied and Environmental Microbiology, June 2004, p. 3769-3771, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3769-3771.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Enhancement of Cry19Aa Mosquitocidal Activity against Aedes aegypti by Mutations in the Putative Loop Regions of Domain II

Mohd Amir F. Abdullah1,2 and Donald H. Dean1*

Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210-1292,1 Department of Science, Faculty of Engineering, International Islamic University Malaysia, Jalan Gombak, 53100 Kuala Lumpur, Malaysia2

Received 25 September 2003/ Accepted 18 February 2004

Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or Culex.

* Corresponding author. Mailing address: Department of Biochemistry, The Ohio State University, 484 W. 12th Ave., Columbus, OH 43210. Phone: (614) 292-8829. Fax: (614) 292-3206. E-mail: dean.10{at}osu.edu.

Applied and Environmental Microbiology, June 2004, p. 3769-3771, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3769-3771.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pigott, C. R., King, M. S., Ellar, D. J. (2008). Investigating the Properties of Bacillus thuringiensis Cry Proteins with Novel Loop Replacements Created Using Combinatorial Molecular Biology. Appl. Environ. Microbiol. 74: 3497-3511 [Abstract] [Full Text]  
  • Liu, X. S., Dean, D. H. (2006). Redesigning Bacillus thuringiensis Cry1Aa toxin into a mosquito toxin. Protein Eng Des Sel 19: 107-111 [Abstract] [Full Text]  


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