This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, X.
Right arrow Articles by Deng, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, X.
Right arrow Articles by Deng, Z.
Agricola
Right arrow Articles by Li, X.
Right arrow Articles by Deng, Z.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2004, p. 3893-3897, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.3893-3897.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of Micromonospora Phage {Phi}HAU8 and Development into a Phasmid

Xiaohua Li,1,2 Xiufen Zhou,1,2 and Zixin Deng1,2*

Bio-X Life Science Research Center and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030,1 School of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China2

Received 25 December 2003/ Accepted 17 March 2004

{Phi}HAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The {Phi}HAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. {Phi}HAU8 was most stable at 4°C in Difco nutrient broth within a pH range of 6 to 12. {Phi}HAU8 plaque formation on Micromonospora sp. strain 40027 was optimal with 32 mM Ca2+ and 30 mM Mg2+. A lysogen, LXH8, was isolated from turbid plaques, and a phasmid derivative that functions as a {lambda} cosmid vector in Escherichia coli and as a phage in Micromonospora sp. strain 40027 was constructed. Pulsed-field gel electrophoresis of AseI-digested total DNA showed that {Phi}HAU8 DNA integrates into the 500-kb AseI fragment of Micromonospora sp. strain 40027.

* Corresponding author. Mailing address: Bio-X Life Science Research Center, Shanghai Jiaotong University, Shanghai 200030, People's Republic of China. Phone: 86 21 62933404. Fax: 86 21 62932418. E-mail: zxdeng{at}sjtu.edu.cn.

Applied and Environmental Microbiology, July 2004, p. 3893-3897, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.3893-3897.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»