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Applied and Environmental Microbiology, July 2004, p. 3968-3972, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.3968-3972.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Relationship between gyrA Mutations and Quinolone Resistance in Flavobacterium psychrophilum Isolates

Shotaro Izumi1* and Futoshi Aranishi1,2

Molecular Biology Division, National Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648,1 Department of Biological Production and Environmental Science, Miyazaki University, Miyazaki 889-2192, Japan2

Received 1 February 2004/ Accepted 16 March 2004

Flavobacterium psychrophilum is the causative agent of the fish diseases called bacterial cold-water disease and rainbow trout fry syndrome. It has been reported that some isolates of F. psychrophilum are resistant to quinolones; however, the mechanism of this quinolone resistance has been unexplained. In this study, we examined the quinolone susceptibility patterns of 27 F. psychrophilum strains isolated in Japan and the United States. Out of 27 isolates, 14 were resistant to both nalidixic acid (NA) and oxolinic acid (OXA), and the others were susceptible to NA and OXA. When amino acid sequences deduced from gyrA nucleotide sequences of all isolates tested were analyzed, two amino acid substitutions (a threonine residue replaced by an alanine or isoleucine residue in position 83 of GyrA [Escherichia coli numbering] and an aspartic acid residue replaced by a tyrosine residue in position 87) were observed in the 14 quinolone-resistant isolates. These results strongly suggest that, as in other gram-negative bacteria, DNA gyrase is an important target for quinolones in F. psychrophilum.

* Corresponding author. Mailing address: Molecular Biology Division, National Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama, Kanagawa 236-8648, Japan. Phone: 81-45-788-7645. Fax: 81-45-788-5001. E-mail: sizumi{at}affrc.go.jp or s-izumi{at}aa.e-mansion.com.

Applied and Environmental Microbiology, July 2004, p. 3968-3972, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.3968-3972.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Spigaglia, P., Barbanti, F., Louie, T., Barbut, F., Mastrantonio, P. (2009). Molecular Analysis of the gyrA and gyrB Quinolone Resistance-Determining Regions of Fluoroquinolone-Resistant Clostridium difficile Mutants Selected In Vitro. Antimicrob. Agents Chemother. 53: 2463-2468 [Abstract] [Full Text]  
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