This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salles, J. F.
Right arrow Articles by van Elsas, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salles, J. F.
Right arrow Articles by van Elsas, J. D.
Agricola
Right arrow Articles by Salles, J. F.
Right arrow Articles by van Elsas, J. D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2004, p. 4012-4020, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4012-4020.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multivariate Analyses of Burkholderia Species in Soil: Effect of Crop and Land Use History

Joana Falcão Salles,1* Johannes Antonius van Veen,2,3 and Jan Dirk van Elsas4

Plant Research International, 6700 AA Wageningen,1 Biological Center, Groningen University, 9750 RA Haren,2 NIOO-CTO, 6666 ZG Heteren,3 Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands4

Received 18 November 2003/ Accepted 5 April 2004

The assessment of Burkholderia diversity in agricultural areas is important considering the potential use of this genus for agronomic and environmental applications. Therefore, the aim of this work was to ascertain how plant species and land use management drive the diversity of the genus Burkholderia. In a greenhouse experiment, different crops, i.e., maize, oat, barley, and grass, were planted in pots containing soils with different land use histories, i.e., maize monoculture, crop rotation, and permanent grassland, for three consecutive growth cycles. The diversity of Burkholderia spp. in the rhizosphere soil was assessed by genus-specific PCR-denaturing gradient gel electrophoresis (DGGE) and analyzed by canonical correspondence analysis (CCA). CCA ordination plots showed that previous land use was the main factor affecting the composition of the Burkholderia community. Although most variation in the Burkholderia community structure was observed between the permanent grassland and agricultural areas, differences between the crop rotation and maize monoculture groups were also observed. Plant species affected Burkholderia community structure to a lesser extent than did land use history. Similarities were observed between Burkholderia populations associated with maize and grass, on the one hand, and between those associated with barley and oat, on the other hand. Additionally, CCA ordination plots demonstrated that these two groups (maize/grass versus barley/oat) had a negative correlation. The identification of bands from the DGGE patterns demonstrated that the species correlated with the environmental variables were mainly affiliated with Burkholderia species that are commonly isolated from soil, in particular Burkholderia glathei, B. caledonica, B. hospita, and B. caribiensis.

* Corresponding author. Mailing address: Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands. Phone: 31 317 475856. Fax: 31 317 410113. E-mail: joana.salles{at}wur.nl.

Applied and Environmental Microbiology, July 2004, p. 4012-4020, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4012-4020.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Onofre-Lemus, J., Hernandez-Lucas, I., Girard, L., Caballero-Mellado, J. (2009). ACC (1-Aminocyclopropane-1-Carboxylate) Deaminase Activity, a Widespread Trait in Burkholderia Species, and Its Growth-Promoting Effect on Tomato Plants. Appl. Environ. Microbiol. 75: 6581-6590 [Abstract] [Full Text]  
  • Andreote, F. D., de Araujo, W. L., de Azevedo, J. L., van Elsas, J. D., da Rocha, U. N., van Overbeek, L. S. (2009). Endophytic Colonization of Potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities. Appl. Environ. Microbiol. 75: 3396-3406 [Abstract] [Full Text]  
  • Bremer, C., Braker, G., Matthies, D., Reuter, A., Engels, C., Conrad, R. (2007). Impact of Plant Functional Group, Plant Species, and Sampling Time on the Composition of nirK-Type Denitrifier Communities in Soil. Appl. Environ. Microbiol. 73: 6876-6884 [Abstract] [Full Text]  
  • van der Peet-Schwering, C. M. C., Jansman, A. J. M., Smidt, H., Yoon, I. (2007). Effects of yeast culture on performance, gut integrity, and blood cell composition of weanling pigs. J ANIM SCI 85: 3099-3109 [Abstract] [Full Text]  
  • Caballero-Mellado, J., Onofre-Lemus, J., Estrada-de los Santos, P., Martinez-Aguilar, L. (2007). The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation. Appl. Environ. Microbiol. 73: 5308-5319 [Abstract] [Full Text]  
  • Nakatsu, C. H. (2007). Soil Microbial Community Analysis Using Denaturing Gradient Gel Electrophoresis. Soil Sci. 71: 562-571 [Abstract] [Full Text]  
  • Tolli, J., King, G. M. (2005). Diversity and Structure of Bacterial Chemolithotrophic Communities in Pine Forest and Agroecosystem Soils. Appl. Environ. Microbiol. 71: 8411-8418 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»