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Applied and Environmental Microbiology, July 2004, p. 4030-4034, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4030-4034.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Salmonella enterica Serovar Typhimurium from Marine Environments in Coastal Waters of Galicia (Spain)

Jaime Martinez-Urtaza,1* Ernesto Liebana,2 Lourdes Garcia-Migura,2 Pelayo Perez-Piñeiro,1 and Montserrat Saco3

Unidad de Control de Moluscos, Instituto de Acuicultura, Universidad de Santiago de Compostela, Campus Universitario Sur, 15782 Santiago de Compostela,1 Laboratori de Sanitat Ramadera de Barcelona, Departamento de Agricultura, Ganaderia y Pesca, Generalitat de Catalunya, Zona Franca, 08040 Barcelona, Spain,3 Department of Food and Environmental Safety, Veterinary Laboratories Agency-Weybridge, Addlestone, Surrey KT15 3NB, United Kingdom2

Received 19 January 2004/ Accepted 5 April 2004

Twenty-three Salmonella enterica serovar Typhimurium isolates from marine environments were characterized by phage typing, pulsed-field gel electrophoresis (PFGE) analysis, plasmid analysis, and antibiotic resistance, and the distribution of the different types in the coastal waters were subsequently analyzed. Five phage types were identified among the isolates (PT41, PT135, PT99, DT104, and DT193). PT135 isolates were exclusively detected during the winter months from 1998 to 2000, whereas DT104 and PT41 isolates were detected exclusively in the summer months from 2000 to 2002. XbaI PFGE analysis revealed 9 PFGE types, and plasmid profiling identified 8 plasmid types (with 1 to 6 plasmids) among the isolates. Only three isolates presented multidrug resistance to antibiotics. Two DT104 isolates were resistant to 8 and 7 antibiotics (profiles ACCeFNaSSuT and ACeFNeSSuT), whereas a PT193 isolate presented resistance to 6 antibiotics (profile ACFSSu). In addition, four PT41 isolates were resistant to a single antibiotic. The detection of multidrug-resistant phage types DT104 and DT193 in shellfish emphasizes the importance of monitoring the presence of Salmonella in routine surveillance of live bivalve molluscs.

* Corresponding author. Mailing address: Unidad de Control de Moluscos, Instituto de Acuicultura, Universidad de Santiago de Compostela, Campus Universitario Sur, 15782 Santiago de Compostela, Spain. Phone: 34 981 563 100, ext. 16043. Fax: 34 981 547 165. E-mail: ucmjmur{at}usc.es.

Applied and Environmental Microbiology, July 2004, p. 4030-4034, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4030-4034.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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