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Applied and Environmental Microbiology, July 2004, p. 4064-4072, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4064-4072.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Single Species, Micromonas pusilla (Prasinophyceae), Dominates the Eukaryotic Picoplankton in the Western English Channel

Fabrice Not,1* Mikel Latasa,2 Dominique Marie,1 Thierry Cariou,1 Daniel Vaulot,1 and Nathalie Simon1

Station Biologique, UMR7127 CNRS, INSU and Université Pierre et Marie Curie, 29680 Roscoff, France,1 Institut de Ciències del Mar (C.S.I.C.), 08003 Barcelona, Spain2

Received 17 September 2003/ Accepted 11 March 2004

The class Prasinophyceae (Chlorophyta) contains several photosynthetic picoeukaryotic species described from cultured isolates. The ecology of these organisms and their contributions to the picoeukaryotic community in aquatic ecosystems have received little consideration. We have designed and tested eight new 18S ribosomal DNA oligonucleotide probes specific for different Prasinophyceae clades, genera, and species. Using fluorescent in situ hybridization associated with tyramide signal amplification, these probes, along with more general probes, have been applied to samples from a marine coastal site off Roscoff (France) collected every 2 weeks between July 2000 and September 2001. The abundance of eukaryotic picoplankton remained high (>103 cells ml–1) during the sampling period, with maxima in summer (up to 2 x 104 cells ml–1), and a single green algal species, Micromonas pusilla (Prasinophyceae), dominated the community all year round. Members of the order Prasinococcales and the species Bathycoccus prasinos (Mamiellales) displayed sporadic occurrences, while the abundances of all other Prasinophyceae groups targeted remained negligible.

* Corresponding author. Mailing address: Station Biologique, UMR 7127, CNRS et Université Pierre et Marie Curie, Place George Teissier, BP74, 29682 Roscoff Cedex, France. Phone: 33 2-98-29-23-70. Fax: 33 2-98-29-23-24. E-mail: vaulot{at}sb-roscoff.fr.

Applied and Environmental Microbiology, July 2004, p. 4064-4072, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4064-4072.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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