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Applied and Environmental Microbiology, July 2004, p. 4073-4078, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4073-4078.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Structural Characterization of Lignin during Pinus taeda Wood Treatment with Ceriporiopsis subvermispora

Anderson Guerra,1 Régis Mendonça,1 André Ferraz,1* Fachuang Lu,2 and John Ralph2

Departamento de Biotecnologia, Faculdade de Engenharia Química de Lorena, 12600-000 Lorena, São Paulo, Brazil,1 U.S. Dairy Forage Research Center, Agricultural Research Service, U.S. Department of Agriculture, Madison, Wisconsin 53706-11082

Received 9 January 2004/ Accepted 29 March 2004

Pinus taeda wood chips were biotreated with Ceriporiopsis subvermispora under solid-state fermentation for periods varying from 15 to 90 days. Milled wood lignins extracted from sound and biotreated wood samples were characterized by wet-chemical and spectroscopic techniques. Treatment of the lignins by derivatization followed by reductive cleavage (DFRC) made it possible to detect DFRC monomers and dimers that are diagnostic of the occurrence of arylglycerol-ß-O-aryl and ß-ß, ß-5, ß-1, and 4-O-5 units in the lignin structure. Quantification of these DFRC products indicated that ß-O-aryl cleavage was a significant route for lignin biodegradation but that ß-ß, ß-5, ß-1, and 4-O-5 linkages were more resistant to the biological attack. The amount of aromatic hydroxyls did not increase with the split of ß-O-4 linkages, suggesting that the ß-O-4 cleavage products remain as quinone-type structures as detected by UV and visible spectroscopy. Nuclear magnetic resonance techniques also indicated the formation of new substructures containing nonoxygenated, saturated aliphatic carbons (CH2 and CH3) in the side chains of lignins extracted from biotreated wood samples.

* Corresponding author. Mailing address: Departamento de Biotecnologia, Faculdade de Engenharia Química de Lorena, CP116, 12600-000 Lorena, São Paulo, Brazil. Phone: 55 12 31595018. Fax: 55 12 31533165. E-mail: aferraz{at}debiq.faenquil.br.

Applied and Environmental Microbiology, July 2004, p. 4073-4078, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4073-4078.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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