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Light Dependence of [³H]Leucine Incorporation in the Oligotrophic North Pacific Ocean

School of Marine Science, The College of William and Mary, Gloucester Point, Virginia 23062,¹ School of Ocean and Earth Science and Technology, University of Hawaii, Honolulu, Hawaii 96822²

Received 26 November 2003/ Accepted 11 March 2004

The influence of irradiance on bacterial incorporation of [³H]leucine was evaluated at Station ALOHA in the oligotrophic North Pacific subtropical gyre. Six experiments were conducted on three cruises to Station ALOHA to examine how [³H]leucine incorporation varied as a function of irradiance. Two experiments were also conducted to assess the photoautotrophic response to irradiance (based on photosynthetic uptake of [¹⁴C]bicarbonate) in both the upper and lower photic zones. Rates of [³H]leucine incorporation responded to irradiance in a photosynthesis-like manner, increasing sharply at low light and then saturating and sometimes declining with increasing light intensity. The influence of irradiance on bacterial growth was evaluated in both the well-lit (5 to 25 m) and dimly lit regions of the upper ocean (75 to 100 m) to determine whether the bacterial response to irradiance differed along the depth-dependent light gradient of the photic zone. [³H]leucine incorporation rates were analyzed with a photosynthesis-irradiance model for a quantitative description of the relationships between [³H]leucine incorporation and irradiance. Maximum rates of [³H]leucine incorporation in the upper photic zone increased 48 to 92% relative to those of dark-incubated samples, with [³H]leucine incorporation saturating at light intensities between 58 and 363 µmol of quanta m^–2 s^–1. Rates of [³H]leucine incorporation in the deep photic zone were photostimulated 53 to 114% and were susceptible to photoinhibition, with rates declining at light intensities of >100 µmol of quanta m^–2 s^–1. The results of these experiments revealed that sunlight directly influences bacterial growth in this open-ocean ecosystem.

This is School of Ocean and Earth Science (SOEST) contribution 6399 and Joint Global Ocean Flux Study (JGOFS) contribution 1045.

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