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Applied and Environmental Microbiology, July 2004, p. 4151-4157, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4151-4157.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effect of Substratum Surface Chemistry and Surface Energy on Attachment of Marine Bacteria and Algal Spores

Linnea K. Ista,¹ Maureen E. Callow,² John A. Finlay,² Sarah E. Coleman,¹ Aleece C. Nolasco,¹ Robin H. Simons,¹ James A. Callow,² and Gabriel P. Lopez^1*

Department of Chemical and Nuclear Engineering, The University of New Mexico, Albuquerque, New Mexico 87131,¹ School of Biosciences, The University of Birmingham, Birmingham B15 2TT, United Kingdom²

Received 13 November 2003/ Accepted 8 March 2004

Two series of self-assembled monolayers (SAMs) of -substituted alkanethiolates on gold were used to systematically examine the effects of varying substratum surface chemistry and energy on the attachment of two model organisms of interest to the study of marine biofouling, the bacterium Cobetia marina (formerly Halomonas marina) and zoospores of the alga Ulva linza (formerly Enteromorpha linza). SAMs were formed on gold-coated glass slides from solutions containing mixtures of methyl- and carboxylic acid-terminated alkanethiols and mixtures of methyl- and hydroxyl-terminated alkanethiols. C. marina attached in increasing numbers to SAMs with decreasing advancing water contact angles (_AW), in accordance with equation-of-state models of colloidal attachment. Previous studies of Ulva zoospore attachment to a series of mixed methyl- and hydroxyl-terminated SAMs showed a similar correlation between substratum _AW and zoospore attachment. When the hydrophilic component of the SAMs was changed to carboxylate, however, the profile of attachment of Ulva was significantly different, suggesting that a more complex model of interfacial energetics is required.

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* Corresponding author. Mailing address: Department of Chemical and Nuclear Engineering, The University of New Mexico, Albuquerque, NM 87131. Phone: (505) 277-4939. Fax: (505) 277-5433. E-mail: gplopez{at}unm.edu.

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Applied and Environmental Microbiology, July 2004, p. 4151-4157, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4151-4157.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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