This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dunfield, K. E.
Right arrow Articles by King, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dunfield, K. E.
Right arrow Articles by King, G. M.
Agricola
Right arrow Articles by Dunfield, K. E.
Right arrow Articles by King, G. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2004, p. 4242-4248, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4242-4248.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of Carbon Monoxide-Oxidizing Bacteria Associated with Recent Hawaiian Volcanic Deposits{dagger}

Kari E. Dunfield and Gary M. King*

Darling Marine Center, University of Maine, Walpole, Maine 04573

Received 14 November 2003/ Accepted 2 April 2004

Genomic DNA extracts from four sites at Kilauea Volcano were used as templates for PCR amplification of the large subunit (coxL) of aerobic carbon monoxide dehydrogenase. The sites included a 42-year-old tephra deposit, a 108-year-old lava flow, a 212-year-old partially vegetated ash-and-tephra deposit, and an approximately 300-year-old forest. PCR primers amplified coxL sequences from the OMP clade of CO oxidizers, which includes isolates such as Oligotropha carboxidovorans, Mycobacterium tuberculosis, and Pseudomonas thermocarboxydovorans. PCR products were used to create clone libraries that provide the first insights into the diversity and phylogenetic affiliations of CO oxidizers in situ. On the basis of phylogenetic and statistical analyses, clone libraries for each site were distinct. Although some clone sequences were similar to coxL sequences from known organisms, many sequences appeared to represent phylogenetic lineages not previously known to harbor CO oxidizers. On the basis of average nucleotide diversity and average pairwise difference, a forested site supported the most diverse CO-oxidizing populations, while an 1894 lava flow supported the least diverse populations. Neither parameter correlated with previous estimates of atmospheric CO uptake rates, but both parameters correlated positively with estimates of microbial biomass and respiration. Collectively, the results indicate that the CO oxidizer functional group associated with recent volcanic deposits of the remote Hawaiian Islands contains substantial and previously unsuspected diversity.

* Corresponding author. Mailing address: Darling Marine Center, University of Maine, Walpole, ME 04573. Phone: (207) 563-3146, ext. 207. Fax: (207) 563-3119. E-mail: gking{at}maine.edu.

{dagger} Contribution no. 393 from the Darling Marine Center.

Applied and Environmental Microbiology, July 2004, p. 4242-4248, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4242-4248.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Smith, J. M., Ogram, A. (2008). Genetic and Functional Variation in Denitrifier Populations along a Short-Term Restoration Chronosequence. Appl. Environ. Microbiol. 74: 5615-5620 [Abstract] [Full Text]  
  • Schloss, P. D., Handelsman, J. (2006). Introducing TreeClimber, a Test To Compare Microbial Community Structures. Appl. Environ. Microbiol. 72: 2379-2384 [Abstract] [Full Text]  
  • Tolli, J. D., Sievert, S. M., Taylor, C. D. (2006). Unexpected Diversity of Bacteria Capable of Carbon Monoxide Oxidation in a Coastal Marine Environment, and Contribution of the Roseobacter-Associated Clade to Total CO Oxidation.. Appl. Environ. Microbiol. 72: 1966-1973 [Abstract] [Full Text]  
  • Tolli, J., King, G. M. (2005). Diversity and Structure of Bacterial Chemolithotrophic Communities in Pine Forest and Agroecosystem Soils. Appl. Environ. Microbiol. 71: 8411-8418 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»