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Applied and Environmental Microbiology, July 2004, p. 4349-4355, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4349-4355.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Fur Is Involved in Manganese-Dependent Regulation of mntA (sitA) Expression in Sinorhizobium meliloti

Raúl Platero,1 Lucía Peixoto,2,{dagger} Mark R. O'Brian,3 and Elena Fabiano1*

Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, MEC, Unidad Asociada a la Facultad de Ciencias,1 Laboratorio de Organización y Evolución del Genoma, Facultad de Ciencias, Montevideo, Uruguay,2 Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York 142143

Received 29 March 2004/ Accepted 2 April 2004

Fur is a transcriptional regulator involved in iron-dependent control of gene expression in many bacteria. In this work we analyzed the phenotype of a fur mutant in Sinorhizobium meliloti, an {alpha}-proteobacterium that fixes N2 in association with host plants. We demonstrated that some functions involved in high-affinity iron transport, siderophore production, and iron-regulated outer membrane protein expression respond to iron in a Fur-independent manner. However, manganese-dependent expression of the MntABCD manganese transport system was lost in a fur strain as discerned by constitutive expression of a mntA::gfp fusion reporter gene in the mutant. Thus, Fur directly or indirectly regulates a manganese-dependent function. The data indicate a novel function for a bacterial Fur protein in mediating manganese-dependent regulation of gene expression.

* Corresponding author. Mailing address: Laboratorio de Ecología Microbiana, IIBCE, Av. Italia 3318, Montevideo 11600, Uruguay. Phone: 598 (2) 4871616, ext. 146. Fax: 598 (2) 4875548. E-mail: efabiano{at}iibce.edu.uy.

{dagger} Present address: Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA 19104.

Applied and Environmental Microbiology, July 2004, p. 4349-4355, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4349-4355.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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