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Applied and Environmental Microbiology, July 2004, p. 4356-4362, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4356-4362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Use of Fluorescent Lectin Probes for Analysis of Footprints from Pseudomonas aeruginosa MDC on Hydrophilic and Hydrophobic Glass Substrata

Eduardo Mora Bejarano and René Peter Schneider*

Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, São Paulo 05508-900, Brazil

Received 3 September 2003/ Accepted 3 February 2004

ABSTRACT

Microbial footprints of Pseudomonas aeruginosa MDC attached for 1 h to clean or silanized glass were analyzed with fluorescently labeled lectin probes. Footprint composition varied, depending on cell physiology and substratum surface chemistry. This suggests that substratum physicochemistry affected the structure of cell surfaces of adsorbed organisms.

* Corresponding author. Mailing address: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, CEP 05508-900, São Paulo, São Paulo, Brazil. Phone: 55 11 3091 7745. Fax: 55 11 3091 7354. E-mail: schneide{at}icb.usp.br.

Applied and Environmental Microbiology, July 2004, p. 4356-4362, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4356-4362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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