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SHORT REPORT

Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean

Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, 1790 AB Den Burg, The Netherlands,¹ Max Planck Institute for Marine Microbiology, D-28359 Bremen, Germany²

Received 20 January 2004/ Accepted 19 March 2004

ABSTRACT

The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4',6'-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up L-aspartic acid (19% versus 10%).

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