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Applied and Environmental Microbiology, August 2004, p. 4419-4423, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.4419-4423.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Mating Type Sequences in Asexually Reproducing Fusarium Species

Zoltán Kerényi,1 Antonio Moretti,2 Cees Waalwijk,3 Brigitta Oláh,1 and László Hornok1,4*

Agricultural Biotechnology Center,1 Department of Agricultural Biotechnology and Microbiology, Group of Mycology, Szent István University, Gödöll, Hungary,4 Institute of Sciences of Food Production, CNR, Bari, Italy,2 Business Unit Biointeractions and Plant Health, Plant Research International, Wageningen, The Netherlands3

Received 8 September 2003/ Accepted 24 February 2004

To assess the potential for mating in several Fusarium species with no known sexual stage, we developed degenerate and semidegenerate oligonucleotide primers to identify conserved mating type (MAT) sequences in these fungi. The putative {alpha} and high-mobility-group (HMG) box sequences from Fusarium avenaceum, F. culmorum, F. poae, and F. semitectum were compared to similar sequences that were described previously for other members of the genus. The DNA sequences of the regions flanking the amplified MAT regions were obtained by inverse PCR. These data were used to develop diagnostic primers suitable for the clear amplification of conserved mating type sequences from any member of the genus Fusarium. By using these diagnostic primers, we identified mating types of 122 strains belonging to 22 species of Fusarium. The {alpha} box and the HMG box from the mating type genes are transcribed in F. avenaceum, F. culmorum, F. poae, and F. semitectum. The novelty of the PCR-based mating type identification system that we developed is that this method can be used on a wide range of Fusarium species, which have proven or expected teleomorphs in different ascomycetous genera, including Calonectria, Gibberella, and Nectria.

* Corresponding author. Mailing address: Agricultural Biotechnology Center, Szent-Györgyi A. u 4, 2100 Gödöll, Hungary. Phone: 36 28 526100. Fax: 36 28 526131. E-mail: hornok{at}abc.hu.

Applied and Environmental Microbiology, August 2004, p. 4419-4423, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.4419-4423.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Houbraken, J., Varga, J., Rico-Munoz, E., Johnson, S., Samson, R. A. (2008). Sexual Reproduction as the Cause of Heat Resistance in the Food Spoilage Fungus Byssochlamys spectabilis (Anamorph Paecilomyces variotii). Appl. Environ. Microbiol. 74: 1613-1619 [Abstract] [Full Text]  


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