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Applied and Environmental Microbiology, August 2004, p. 4604-4612, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.4604-4612.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of a Novel Plasma Membrane Protein, Expressed in the Midgut Epithelia of Bombyx mori, That Binds to Cry1A Toxins

Delwar M. Hossain,1 Yasuyuki Shitomi,1 Kenta Moriyama,1 Masahiro Higuchi,1 Tohru Hayakawa,1 Toshiaki Mitsui,1 Ryoichi Sato,2 and Hidetaka Hori1*

Molecular Life Science Laboratory, Graduate School of Science and Technology, Niigata University, Niigata 950-2181,1 Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan2

Received 20 February 2004/ Accepted 21 April 2004

We describe the properties of a novel 252-kDa protein (P252) isolated from brush border membranes of Bombyx mori. P252 was found in a Triton X-100-soluble brush border membrane vesicle fraction, suggesting that it may be a component of the midgut epithelial cell membrane. P252 was purified to homogeneity, and the amino acid sequence of two internal peptides was determined, but neither of the peptides matched protein sequences in the available databases. The apparent molecular mass of the purified protein was estimated by denaturing gel electrophoresis to be 252 kDa, and it migrated as a single band on native gels. However, gel filtration chromatography indicated an apparent mass of 985 kDa, suggesting that P252 may exist as a homo-oligomer. The associations of P252 with Cry1Aa, Cry1Ab, and Cry1Ac were specific, and Kd constants were determined to be 28.9, 178.5, and 20.0 nM, respectively. A heterologous competition assay was also done. P252 did not exhibit Leu-pNA hydrolysis activity, and binding to the Cry1A toxins was not inhibited by GalNAc. Binding assays of P252 with various lectins indicated the presence of three antennal N-linked high-mannose-type as well as O-linked mucin-type sugar side chains. While the function of P252 is not yet clear, we propose that it may function with Cry1A toxins during the insecticidal response and/or Cry toxin resistance mechanism.

* Corresponding author. Mailing address: Graduate School of Science and Technology, Niigata University, Niigata 950-2181, Japan. Phone and fax: 81 25 262 7637. E-mail: hide-hri{at}gs.niigata-u.ac.jp.

Applied and Environmental Microbiology, August 2004, p. 4604-4612, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.4604-4612.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pandian, G. N., Ishikawa, T., Togashi, M., Shitomi, Y., Haginoya, K., Yamamoto, S., Nishiumi, T., Hori, H. (2008). Bombyx mori Midgut Membrane Protein P252, Which Binds to Bacillus thuringiensis Cry1A, Is a Chlorophyllide-Binding Protein, and the Resulting Complex Has Antimicrobial Activity. Appl. Environ. Microbiol. 74: 1324-1331 [Abstract] [Full Text]  
  • Pigott, C. R., Ellar, D. J. (2007). Role of Receptors in Bacillus thuringiensis Crystal Toxin Activity. Microbiol. Mol. Biol. Rev. 71: 255-281 [Abstract] [Full Text]  
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