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Applied and Environmental Microbiology, August 2004, p. 5030-5032, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5030-5032.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Nisin-Controlled Production of Pediocin PA-1 and Colicin V in Nisin- and Non-Nisin-Producing Lactococcus lactis Strains

Nikki Horn,1 Antonio Fernández,1,2 Helen M. Dodd,1 Michael J. Gasson,1 and Juan M. Rodríguez2*

Food Safety Science Division, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom,1 Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain2

Received 19 December 2003/ Accepted 5 May 2004

ABSTRACT

The introduction of chimeric genes encoding the fusion leader of lactococcin A-propediocin PA-1 or procolicin V under the control of the inducible nisA promoter and the lactococcin A-dedicated secretion genes (lcnCD) into Lactococcus lactis strains, including a nisin producer, expressing the two component regulator NisRK led to the production or pediocin PA-1 or colicin V, respectively.

* Corresponding author. Mailing address: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. Phone: 34 91 3943747. Fax: 34 91 3943743. E-mail: jmrodrig{at}vet.ucm.es.

Applied and Environmental Microbiology, August 2004, p. 5030-5032, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5030-5032.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.