AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Valente, C. A.
Right arrow Articles by Monteiro, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Valente, C. A.
Right arrow Articles by Monteiro, G. A.
Agricola
Right arrow Articles by Valente, C. A.
Right arrow Articles by Monteiro, G. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2004, p. 5033-5036, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5033-5036.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Translational Features of Human Alpha 2b Interferon Production in Escherichia coli

C. A. Valente, D. M. F. Prazeres, J. M. S. Cabral, and G. A. Monteiro*

Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisbon, Portugal

Received 21 November 2003/ Accepted 29 April 2004

ABSTRACT

The yield of human alpha 2b interferon in Escherichia coli was optimized by replacement of low-usage arginine codons located in the mRNA 5' end. The differences observed among the various gene variants suggest that codon usage, Shine-Dalgarno-like sequences, and mRNA secondary structure contribute to the performance of E. coli translation machinery.

* Corresponding author. Mailing address: Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. Phone: 351-218419065. Fax: 351-218419062. E-mail: gabmonteiro{at}ist.utl.pt.

Applied and Environmental Microbiology, August 2004, p. 5033-5036, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5033-5036.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2004 by the American Society for Microbiology. All rights reserved.