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Applied and Environmental Microbiology, August 2004, p. 5033-5036, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5033-5036.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Translational Features of Human Alpha 2b Interferon Production in Escherichia coli

C. A. Valente, D. M. F. Prazeres, J. M. S. Cabral, and G. A. Monteiro*

Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisbon, Portugal

Received 21 November 2003/ Accepted 29 April 2004

ABSTRACT

The yield of human alpha 2b interferon in Escherichia coli was optimized by replacement of low-usage arginine codons located in the mRNA 5' end. The differences observed among the various gene variants suggest that codon usage, Shine-Dalgarno-like sequences, and mRNA secondary structure contribute to the performance of E. coli translation machinery.

* Corresponding author. Mailing address: Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. Phone: 351-218419065. Fax: 351-218419062. E-mail: gabmonteiro{at}ist.utl.pt.

Applied and Environmental Microbiology, August 2004, p. 5033-5036, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5033-5036.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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