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Applied and Environmental Microbiology, August 2004, p. 5037-5040, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5037-5040.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Direct Production of Ethanol from Raw Corn Starch via Fermentation by Use of a Novel Surface-Engineered Yeast Strain Codisplaying Glucoamylase and {alpha}-Amylase

Hisayori Shigechi,1 Jun Koh,1 Yasuya Fujita,2 Takeshi Matsumoto,2 Yohei Bito,1 Mitsuyoshi Ueda,3 Eiichi Satoh,4 Hideki Fukuda,2 and Akihiko Kondo1*

Department of Chemical Science and Engineering, Faculty of Engineering,1 Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501,2 Department of Applied Biochemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502,3 Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan4

Received 24 January 2004/ Accepted 4 May 2004

ABSTRACT

Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis {alpha}-amylase by using the C-terminal-half region of {alpha}-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.

* Corresponding author. Mailing address: Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 6547-8501, Japan. Phone: 81-78-803-6196. Fax: 81-78-803-6196. E-mail: kondo{at}cx.kobe-u.ac.jp.

Applied and Environmental Microbiology, August 2004, p. 5037-5040, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5037-5040.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nevoigt, E. (2008). Progress in Metabolic Engineering of Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 72: 379-412 [Abstract] [Full Text]  
  • Okano, K., Zhang, Q., Kimura, S., Narita, J., Tanaka, T., Fukuda, H., Kondo, A. (2008). System Using Tandem Repeats of the cA Peptidoglycan-Binding Domain from Lactococcus lactis for Display of both N- and C-Terminal Fusions on Cell Surfaces of Lactic Acid Bacteria. Appl. Environ. Microbiol. 74: 1117-1123 [Abstract] [Full Text]  
  • Narita, J., Okano, K., Kitao, T., Ishida, S., Sewaki, T., Sung, M.-H., Fukuda, H., Kondo, A. (2006). Display of {alpha}-Amylase on the Surface of Lactobacillus casei Cells by Use of the PgsA Anchor Protein, and Production of Lactic Acid from Starch. Appl. Environ. Microbiol. 72: 269-275 [Abstract] [Full Text]  


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