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Applied and Environmental Microbiology, August 2004, p. 5041-5046, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5041-5046.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Temporal Transcription Map of the Virulent Streptococcus thermophilus Bacteriophage Sfi19

Marco Ventura and Harald Brüssow*

Nestlé Research Center, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland

Received 10 February 2004/ Accepted 21 April 2004

ABSTRACT

A transcription map was developed for the virulent Streptococcus thermophilus phage Sfi19 on the basis of systematic Northern blot hybridizations. All deduced 5' ends were confirmed by primer extension experiments. Three classes of transcripts were detected based on the different times of appearance. Early transcripts were identified in three genome regions; middle transcripts covered cro-like, DNA replication, and transcriptional regulation genes; and late genes consisted of structural and lysis genes. Chloramphenicol treatment suppressed the translation of a putative transcriptional factor necessary for the production of late transcripts and shifted middle transcripts to early transcription times.

* Corresponding author. Mailing address: Nestlé Research Center, Nestec Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland. Phone: 41-21-785-8111. Fax: 41-21-785-8549. E-mail: harald.bruessow{at}rdls.nestle.com.

Applied and Environmental Microbiology, August 2004, p. 5041-5046, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5041-5046.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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