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Applied and Environmental Microbiology, August 2004, p. 5051-5053, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5051-5053.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Nisin-Producing Lactococcus lactis Strains Isolated from Human Milk

Shea S. Beasley and Per E. J. Saris*

Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, Finland

Received 17 December 2003/ Accepted 20 April 2004

ABSTRACT

Characterization by partial 16S rRNA gene sequencing, ribotyping, and green fluorescent protein-based nisin bioassay revealed that 6 of 20 human milk samples contained nisin-producing Lactococcus lactis bacteria. This suggests that the history of humans consuming nisin is older than the tradition of consuming fermented milk products.


* Corresponding author. Mailing address: Department of Applied Chemistry and Microbiology, Biocenter 1, Viikinkaari 9, FIN-00014 University of Helsinki, Finland. Phone: 358-9-19159369. Fax: 385-9-19159322. E-mail: per.saris{at}helsinki.fi.


Applied and Environmental Microbiology, August 2004, p. 5051-5053, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.5051-5053.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




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