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Applied and Environmental Microbiology, September 2004, p. 5089-5093, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5089-5093.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Calicivirus Inactivation by Nonionizing (253.7-Nanometer-Wavelength [UV]) and Ionizing (Gamma) Radiation

Microbiological Laboratory for Health Protection,¹ Diagnostic Laboratory for Infectious Diseases, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands,² Hygiene Institute, University of Vienna,³ Institute of Medical Physics and Biostatistics, University of Veterinary Medicine, Vienna,⁴ Austrian Research Center, Seibersdorf, Austria⁵

Received 31 October 2003/ Accepted 10 May 2004

Noroviruses (previously Norwalk-like viruses) are the most common viral agents associated with food- and waterborne outbreaks of gastroenteritis. In the absence of culture methods for noroviruses, animal caliciviruses were used as model viruses to study inactivation by nonionizing (253.7-nm-wavelength [UV]) and ionizing (gamma) radiation. Here, we studied the respiratory feline calicivirus (FeCV) and the presumed enteric canine calicivirus (CaCV) and compared them with the well-studied bacteriophage MS2. When UV irradiation was used, a 3-log₁₀ reduction was observed at a fluence of 120 J/m² in the FeCV suspension and at a fluence of 200 J/m² for CaCV; for the more resistant phage MS2 there was a 3-log₁₀ reduction at a fluence of 650 J/m². Few or no differences were observed between levels of UV inactivation in high- and low-protein-content virus stocks. In contrast, ionizing radiation could readily inactivate MS2 in water, and there was a 3-log₁₀ reduction at a dose of 100 Gy, although this did not occur when the phage was diluted in high-protein-content stocks of CaCV or FeCV. The low-protein-content stocks showed 3-log₁₀ reductions at a dose of 500 Gy for FeCV and at a dose of 300 for CaCV. The inactivation rates for both caliciviruses with ionizing and nonionizing radiation were comparable but different from the inactivation rates for MS2. Although most FeCV and CaCV characteristics, such as overall particle and genome size and structure, are similar, the capsid sequences differ significantly, making it difficult to predict human norovirus inactivation. Adequate management of UV and gamma radiation processes for virus inactivation should limit public health risks.

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