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Applied and Environmental Microbiology, September 2004, p. 5336-5342, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5336-5342.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effect of Forage or Grain Diets with or without Monensin on Ruminal Persistence and Fecal Escherichia coli O157:H7 in Cattle{dagger}

M. J. Van Baale,1 J. M. Sargeant,1 D. P. Gnad,2 B. M. DeBey,3 K. F. Lechtenberg,4 and T. G. Nagaraja3*

Food Animal Health and Management Center,1 Department of Diagnostic Medicine/Pathobiology,3 Department of Clinical Sciences, Kansas State University, Manhattan, Kansas,2 Midwest Veterinary Services, Inc., Oakland, Nebraska4

Received 24 December 2003/ Accepted 16 May 2004

Twelve ruminally cannulated cattle, adapted to forage or grain diet with or without monensin, were used to investigate the effects of diet and monensin on concentration and duration of ruminal persistence and fecal shedding of E. coli O157:H7. Cattle were ruminally inoculated with a strain of E. coli O157:H7 (1010 CFU/animal) made resistant to nalidixic acid (Nalr). Ruminal and fecal samples were collected for 11 weeks, and then cattle were euthanized and necropsied and digesta from different gut locations were collected. Samples were cultured for detection and enumeration of Nalr E. coli O157:H7. Cattle fed forage diets were culture positive for E. coli O157:H7 in the feces for longer duration (P < 0.05) than cattle fed a grain diet. In forage-fed cattle, the duration they remained culture positive for E. coli O157:H7 was shorter (P < 0.05) when the diet included monensin. Generally, ruminal persistence of Nalr E. coli O157:H7 was not affected by diet or monensin. At necropsy, E. coli O157:H7 was detected in cecal and colonic digesta but not from the rumen. Our study showed that cattle fed a forage diet were culture positive longer and with higher numbers than cattle on a grain diet. Monensin supplementation decreased the duration of shedding with forage diet, and the cecum and colon were culture positive for E. coli O157:H7 more often than the rumen of cattle.

* Corresponding author. Mailing address: Department of Diagnostic Medicine/Pathobiology, 305 Coles Hall, Kansas State University, Manhattan, KS 66506-5606. Phone: (785) 532-1214. Fax: (785) 532-4851. E-mail: tnagaraj{at}vet.ksu.edu.

{dagger} This paper is contribution no. 03-228-J from the Kansas Agricultural Experiment Station.

Applied and Environmental Microbiology, September 2004, p. 5336-5342, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5336-5342.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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