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Applied and Environmental Microbiology, September 2004, p. 5522-5527, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5522-5527.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Phylogenetic Analysis of Polyketide Synthase I Domains from Soil Metagenomic Libraries Allows Selection of Promising Clones

Aurélien Ginolhac,1,2* Cyrille Jarrin,1,2 Benjamin Gillet,1 Patrick Robe,1 Petar Pujic,1 Karine Tuphile,1 Hélène Bertrand,2 Timothy M. Vogel,2 Guy Perrière,3 Pascal Simonet,2 and Renaud Nalin1

LibraGen S.A.,1 Écologie Microbienne, UMR CNRS 5557,2 Laboratoire de Biométrie et Biologie Évolutive, UMR CNRS 5558, Université Claude Bernard, Villeurbanne, France3

Received 6 May 2004/ Accepted 10 May 2004

The metagenomic approach provides direct access to diverse unexplored genomes, especially from uncultivated bacteria in a given environment. This diversity can conceal many new biosynthetic pathways. Type I polyketide synthases (PKSI) are modular enzymes involved in the biosynthesis of many natural products of industrial interest. Among the PKSI domains, the ketosynthase domain (KS) was used to screen a large soil metagenomic library containing more than 100,000 clones to detect those containing PKS genes. Over 60,000 clones were screened, and 139 clones containing KS domains were detected. A 700-bp fragment of the KS domain was sequenced for 40 of 139 randomly chosen clones. None of the 40 protein sequences were identical to those found in public databases, and nucleic sequences were not redundant. Phylogenetic analyses were performed on the protein sequences of three metagenomic clones to select the clones which one can predict to produce new compounds. Two PKS-positive clones do not belong to any of the 23 published PKSI included in the analysis, encouraging further analyses on these two clones identified by the selection process.

* Corresponding author. Mailing address: LibraGen S.A., BÂtiment Canal Biotech 1, 3 rue des Satellites, 31400 Toulouse, France. Phone: 33 (0) 5 62 19 32 90. Fax: 33 (0) 5 61 73 27 56. E-mail: contact{at}libragen.com.

Applied and Environmental Microbiology, September 2004, p. 5522-5527, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5522-5527.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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