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Applied and Environmental Microbiology, September 2004, p. 5589-5594, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5589-5594.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Blakeslea trispora Genes for Carotene Biosynthesis

M. Rodríguez-Sáiz, B. Paz, J. L. de la Fuente, M. J. López-Nieto, W. Cabri, and J. L. Barredo*

R&D Biology, Antibióticos S. A. and Vitatene, E-24009 León, Spain

Received 2 February 2004/ Accepted 15 May 2004

We cloned the carB and carRA genes involved in ß-carotene biosynthesis from overproducing and wild-type strains of Blakeslea trispora. The carB gene has a length of 1,955 bp, including two introns of 141 and 68 bp, and encodes a protein of 66.4 kDa with phytoene dehydrogenase activity. The carRA gene contains 1,894 bp, with a single intron of 70 bp, and encodes a protein of 69.6 kDa with separate domains for lycopene cyclase and phytoene synthase. The estimated transcript sizes for carB and carRA were 1.8 and 1.9 kb, respectively. CarB from the ß-carotene-overproducing strain B. trispora F-744 had an S528R mutation and a TAG instead of a TAA stop codon. The overproducing strain also had a P143S mutation in CarRA. Both B. trispora genes could complement mutations in orthologous genes in Mucor circinelloides and could be used to construct transformed strains of M. circinelloides that produced higher levels of ß-carotene than did the nontransformed parent. The results show that these genes are conserved across the zygomycetes and that the B. trispora carB and carRA genes are functional and potentially useable to increase carotenoid production.

* Corresponding author. Mailing address: R&D Biology, Antibióticos S.A., Avenida de Antibióticos 59-61, E-24009 León, Spain. Phone: 34 (987) 895826. Fax: 34 (987) 895986. E-mail: jbarredo{at}antibioticos.it.

Applied and Environmental Microbiology, September 2004, p. 5589-5594, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5589-5594.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kuzina, V., Cerda-Olmedo, E. (2006). Modification of Sexual Development and Carotene Production by Acetate and Other Small Carboxylic Acids in Blakeslea trispora and Phycomyces blakesleeanus.. Appl. Environ. Microbiol. 72: 4917-4922 [Abstract] [Full Text]  


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