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Applied and Environmental Microbiology, September 2004, p. 5692-5694, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5692-5694.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Fluorogenic Selective and Differential Medium for Isolation of Enterobacter sakazakii

Se-Wook Oh{dagger} and Dong-Hyun Kang*

Department of Food Science and Human Nutrition, Washington State University, Pullman, Washington

Received 2 March 2004/ Accepted 3 May 2004

ABSTRACT

4-Methylumbelliferyl-{alpha}-D-glucoside, the fluorogenic substrate of {alpha}-glucosidase, was used as a selective marker to develop a differential medium for Enterobacter sakazakii. This bacterium showed strong fluorogenic characteristics clearly distinguishable from other microorganisms. On the basis of reducing background noise, an optimum basal medium and nitrogen source were selected. Incubation conditions were optimized.

* Corresponding author. Mailing address: Department of Food Science and Human Nutrition, Washington State University, Pullman, WA 99164-6376. Phone: (509) 335-3937. Fax: (509) 335-4815. E-mail: dhkang{at}wsu.edu.

FOOTNOTES

{dagger} Present address: Korea Food Research Institute, Sungnam-Si 463-420, Korea.

Applied and Environmental Microbiology, September 2004, p. 5692-5694, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5692-5694.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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