This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dick, L. K. Articles by Field, K. G. Search for Related Content PubMed PubMed Citation Articles by Dick, L. K. Articles by Field, K. G. Agricola Articles by Dick, L. K. Articles by Field, K. G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, September 2004, p. 5695-5697, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5695-5697.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Rapid Estimation of Numbers of Fecal Bacteroidetes by Use of a Quantitative PCR Assay for 16S rRNA Genes

Department of Microbiology, Oregon State University, Corvallis, Oregon

Received 25 August 2003/ Accepted 14 May 2004

ABSTRACT

Assessment of health risk associated with fecal pollution requires a reliable fecal indicator and a rapid quantification method. We report the development of a Taq nuclease assay for enumeration of 16S rRNA genes of Bacteroidetes. Sensitivity and correlation with standard fecal indicators provide experimental evidence for application of the assay in monitoring fecal pollution.

This article has been cited by other articles:

• Lu, J., Santo Domingo, J. W., Lamendella, R., Edge, T., Hill, S. (2008). Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces. Appl. Environ. Microbiol. 74: 3969-3976 [Abstract] [Full Text]  
• Hong, P.-Y., Wu, J.-H., Liu, W.-T. (2008). Relative Abundance of Bacteroides spp. in Stools and Wastewaters as Determined by Hierarchical Oligonucleotide Primer Extension. Appl. Environ. Microbiol. 74: 2882-2893 [Abstract] [Full Text]  
• Gourmelon, M., Caprais, M. P., Segura, R., Le Mennec, C., Lozach, S., Piriou, J. Y., Rince, A. (2007). Evaluation of Two Library-Independent Microbial Source Tracking Methods To Identify Sources of Fecal Contamination in French Estuaries. Appl. Environ. Microbiol. 73: 4857-4866 [Abstract] [Full Text]  
• Wu, J.-H., Liu, W.-T. (2007). Quantitative multiplexing analysis of PCR-amplified ribosomal RNA genes by hierarchical oligonucleotide primer extension reaction. Nucleic Acids Res 35: e82-e82 [Abstract] [Full Text]  
• Sinigalliano, C. D., Gidley, M. L., Shibata, T., Whitman, D., Dixon, T. H., Laws, E., Hou, A., Bachoon, D., Brand, L., Amaral-Zettler, L., Gast, R. J., Steward, G. F., Nigro, O. D., Fujioka, R., Betancourt, W. Q., Vithanage, G., Mathews, J., Fleming, L. E., Solo-Gabriele, H. M. (2007). Impacts of Hurricanes Katrina and Rita on the microbial landscape of the New Orleans area. Proc. Natl. Acad. Sci. USA 104: 9029-9034 [Abstract] [Full Text]  
• Stoeckel, D. M., Harwood, V. J. (2007). Performance, Design, and Analysis in Microbial Source Tracking Studies. Appl. Environ. Microbiol. 73: 2405-2415 [Full Text]  
• Shanks, O. C., Nietch, C., Simonich, M., Younger, M., Reynolds, D., Field, K. G. (2006). Basin-Wide Analysis of the Dynamics of Fecal Contamination and Fecal Source Identification in Tillamook Bay, Oregon. Appl. Environ. Microbiol. 72: 5537-5546 [Abstract] [Full Text]  
• Walters, S. P., Field, K. G. (2006). Persistence and growth of fecal bacteroidales assessed by bromodeoxyuridine immunocapture.. Appl. Environ. Microbiol. 72: 4532-4539 [Abstract] [Full Text]  
• Layton, A., McKay, L., Williams, D., Garrett, V., Gentry, R., Sayler, G. (2006). Development of Bacteroides 16S rRNA Gene TaqMan-Based Real-Time PCR Assays for Estimation of Total, Human, and Bovine Fecal Pollution in Water.. Appl. Environ. Microbiol. 72: 4214-4224 [Abstract] [Full Text]  
• Soule, M., Kuhn, E., Loge, F., Gay, J., Call, D. R. (2006). Using DNA microarrays to identify library-independent markers for bacterial source tracking.. Appl. Environ. Microbiol. 72: 1843-1851 [Abstract] [Full Text]  
• Noble, R. T., Griffith, J. F., Blackwood, A. D., Fuhrman, J. A., Gregory, J. B., Hernandez, X., Liang, X., Bera, A. A., Schiff, K. (2006). Multitiered Approach Using Quantitative PCR To Track Sources of Fecal Pollution Affecting Santa Monica Bay, California. Appl. Environ. Microbiol. 72: 1604-1612 [Abstract] [Full Text]  
• Bower, P. A., Scopel, C. O., Jensen, E. T., Depas, M. M., McLellan, S. L. (2005). Detection of Genetic Markers of Fecal Indicator Bacteria in Lake Michigan and Determination of Their Relationship to Escherichia coli Densities Using Standard Microbiological Methods. Appl. Environ. Microbiol. 71: 8305-8313 [Abstract] [Full Text]