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Applied and Environmental Microbiology, September 2004, p. 5701-5703, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5701-5703.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

PCR-Based Identification of Hyperthermophilic Archaea of the Family Thermococcaceae

Galina B. Slobodkina, Nikolai A. Chernyh, Alexander I. Slobodkin, Irina V. Subbotina, Elizaveta A. Bonch-Osmolovskaya, and Alexander V. Lebedinsky*

Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia

Received 14 October 2003/ Accepted 25 May 2004

ABSTRACT

A method for rapid detection and identification of hyperthermophilic archaea of the family Thermococcaceae based on PCR amplification of 16S rRNA gene fragments with primers TcPc 173F (5'-TCCCCCATAGGYCTGRGGTACTGGAAGGTC-3') and TcPc 589R (5'-GCCGTGRGATTTCGCCAGGGACTTACGGGC-3') was developed and used for identification of new isolates.

* Corresponding author. Mailing address: Institute of Microbiology, Russian Academy of Sciences, prospekt 60-letiya Oktyabrya 7/2, Moscow, 117312 Russia. Phone: 7-095-135-4458. Fax: 7-095-135-6530. E-mail: a.lebedinsky{at}mail.ru.

Applied and Environmental Microbiology, September 2004, p. 5701-5703, Vol. 70, No. 9
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.9.5701-5703.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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