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Applied and Environmental Microbiology, January 2005, p. 190-196, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.190-196.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Unveiling of Novel Radiations within Trichodesmium Cluster by hetR Gene Sequence Analysis

Pernilla Lundgren,1 Sven Janson,2* Sara Jonasson,1 Alon Singer,1 and Birgitta Bergman1

Department of Botany, Stockholm University, Stockholm,1 Department of Biology and Environmental Science, University of Kalmar, Kalmar, Sweden2

Received 22 October 2003/ Accepted 10 August 2004

The filamentous nonheterocystous cyanobacterial genus Katagnymene is a common diazotrophic component of tropical and subtropical oceans. To assess the phylogenetic affiliation of this taxon, two partial 16S rRNA gene sequences and 25 partial hetR gene sequences originating from the genera Katagnymene and Trichodesmium collected from open, surface waters of the Atlantic, Indian, and Pacific oceans were compared. Single trichomes or colonies were identified morphologically by using light microscopy and then used directly as templates in hetR PCR analyses. In addition, three cultured strains, identified as Katagnymene pelagica, Katagnymene spiralis, and Trichodesmium sp., were examined. The data show that the genus Katagnymene is in the Trichodesmium cluster and that K. pelagica Lemmermann and K. spiralis Lemmermann are most likely one species, despite their different morphologies. Phylogenetic analyses also unveiled four distinct clusters in the Trichodesmium cluster, including one novel cluster. Our findings emphasize the conclusion that known morphological traits used to differentiate marine nonheterocystous cyanobacteria at the genus and species levels correlate poorly with genetic data, and a revision is therefore suggested.

* Corresponding author. Mailing address: Department of Biology and Environmental Science, University of Kalmar, SE-391 82 Kalmar, Sweden. Phone: 46 480447310. Fax: 46 480447305. E-mail: sven.janson{at}hik.se.

Applied and Environmental Microbiology, January 2005, p. 190-196, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.190-196.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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