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Applied and Environmental Microbiology, January 2005, p. 39-45, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.39-45.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Bacillus subtilis {alpha}-Phosphoglucomutase Is Required for Normal Cell Morphology and Biofilm Formation{dagger}

Vladimir Lazarevic,1* Blazenka Soldo,1 Noël Médico,1 Harold Pooley,1 Sierd Bron,2 and Dimitri Karamata1

Département de Microbiologie Fondamentale, BÂtiment de Biologie, Faculté de Biologie et de Médecine, Université de Lausanne, Lausanne, Switzerland,1 Groningen Biomolecular Sciences and Biotechnology Institute, Department of Genetics, University of Groningen, Haren, The Netherlands2

Received 10 May 2004/ Accepted 6 August 2004

Mutations designated gtaC and gtaE that affect {alpha}-phosphoglucomutase activity required for interconversion of glucose 6-phosphate and {alpha}-glucose 1-phosphate were mapped to the Bacillus subtilis pgcA (yhxB) gene. Backcrossing of the two mutations into the 168 reference strain was accompanied by impaired {alpha}-phosphoglucomutase activity in the soluble cell extract fraction, altered colony and cell morphology, and resistance to phages {phi}29 and {rho}11. Altered cell morphology, reversible by additional magnesium ions, may be correlated with a deficiency in the membrane glycolipid. The deficiency in biofilm formation in gtaC and gtaE mutants may be attributed to an inability to synthesize UDP-glucose, an important intermediate in a number of cell envelope biosynthetic processes.

* Corresponding author. Mailing address: Département de Microbiologie Fondamentale, BÂtiment de Biologie, Faculté de Biologie et de Médecine, Université de Lausanne, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 56 08. Fax: 41 21 692 56 05. E-mail: vladimir.lazarevic{at}imf.unil.ch.

{dagger} Dedicated to the memory of Catherine Mauël (1943-2004).

Applied and Environmental Microbiology, January 2005, p. 39-45, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.39-45.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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