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Applied and Environmental Microbiology, January 2005, p. 507-511, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.507-511.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Legionella Species Diversity in an Acidic Biofilm Community in Yellowstone National Park

Kathy B. Sheehan,1* Joan M. Henson,1 and Michael J. Ferris2

Department of Microbiology and the Thermal Biology Institute, Montana State University, Bozeman, Montana,1 Research Institute for Children, New Orleans, Louisiana2

Received 27 May 2004/ Accepted 20 August 2004

Legionella species are frequently detected in aquatic environments, but their occurrence in extreme, acidic, geothermal habitats has not been explored with cultivation-independent methods. We investigated a predominately eukaryotic algal mat community in a pH 2.7 geothermal stream in Yellowstone National Park for the presence of Legionella and potential host amoebae. Our analyses, using PCR amplification with Legionella-specific primers targeting 16S rRNA genes, detected four known Legionella species, as well as Legionella sequences from species that are not represented in sequence databases, in mat samples and cultivated isolates. The nonrandom occurrence of sequences detected at lower (30°C) and higher (35 to 38°C) temperatures suggests that natural thermal gradients in the stream influence Legionella species distributions in this mat community. We detected only one sequence, Legionella micdadei, from cultivated isolates. We cultured and sequenced partial 18S rRNA gene regions from two potential hosts, Acanthamoeba and Euglena species.

* Corresponding author. Mailing address: Division of Health Sciences, WWAMI Medical Program, 308 Leon Johnson Hall, Montana State University, P.O. Box 173080, Bozeman, MT 59717-3080. Phone: (406) 994-5415. Fax: (406) 994-4398. E-mail: umbks{at}montana.edu.

Applied and Environmental Microbiology, January 2005, p. 507-511, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.507-511.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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