This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by del Campo, R.
Right arrow Articles by Baquero, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by del Campo, R.
Right arrow Articles by Baquero, F.
Agricola
Right arrow Articles by del Campo, R.
Right arrow Articles by Baquero, F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2005, p. 547-549, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.547-549.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Scarce Evidence of Yogurt Lactic Acid Bacteria in Human Feces after Daily Yogurt Consumption by Healthy Volunteers

Rosa del Campo,1* Daniel Bravo,1 Rafael Cantón,1 Patricia Ruiz-Garbajosa,1 Raimundo García-Albiach,2 Alejandra Montesi-Libois,2 Francisco-Javier Yuste,1 Victor Abraira,1 and Fernando Baquero1

Departments of Microbiology, Preventive Medicine, and Biostatistics, Ramón y Cajal University Hospital,1 Department of Microbiology, San Pablo-CEU University, Madrid, Spain2

Received 3 June 2004/ Accepted 24 August 2004

In a double-blind prospective study including 114 healthy young volunteers, the presence in human feces of the yogurt organisms Lactobacillus delbrueckii and Streptococcus thermophilus after repeated yogurt consumption (15 days) was analyzed by culture, specific PCR, and DNA hybridization of total fecal DNA. Detection of yogurt lactic acid bacteria in total fecal DNA by bacterial culture and PCR assay was consistently negative. DNA compatible with yogurt bacteria was found by hybridization experiments in only 10 (10.52%) of 96 individuals after consumption of fresh yogurt and in 2 (2.10%) of 96 individuals after consumption of pasteurized yogurt (P = 0.01).

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Ctra. Colmenar, Km 9.1, Madrid 28.034, Spain. Phone: 34-91 3368542. Fax: 34-91 3368809. E-mail: rosacampo{at}yahoo.com.

Applied and Environmental Microbiology, January 2005, p. 547-549, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.547-549.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Garcia-Albiach, R., Jose, M., de Felipe, P., Angulo, S., Morosini, M.-I., Bravo, D., Baquero, F., del Campo, R. (2008). Molecular analysis of yogurt containing Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in human intestinal microbiota. Am. J. Clin. Nutr. 87: 91-96 [Abstract] [Full Text]  
  • Elli, M., Callegari, M. L., Ferrari, S., Bessi, E., Cattivelli, D., Soldi, S., Morelli, L., Goupil Feuillerat, N., Antoine, J.-M. (2006). Survival of yogurt bacteria in the human gut.. Appl. Environ. Microbiol. 72: 5113-5117 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»