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Applied and Environmental Microbiology, January 2005, p. 558-561, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.558-561.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Transcriptional Analysis of Genes Encoding Shiga Toxin 2 and Its Variants in Escherichia coli

Wenlan Zhang, Martina Bielaszewska, Alexander W. Friedrich, Thorsten Kuczius, and Helge Karch*

Institut für Hygiene, Universitätsklinikum Münster, Münster, Germany

Received 15 March 2004/ Accepted 29 August 2004

Six of 37 non-O157 Escherichia coli strains possessing Shiga toxin (Stx) 2 gene variant stx2d or stx2e secreted no detectable Stx. These isolates produced significantly less stx mRNA than Stx2d, Stx2e, Stx2c, or Stx2 secretors did. Standard screening procedures miss a significant subset of E. coli harboring stx2 variants.

* Corresponding author. Mailing address: Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch-Str. 41, 48149 Münster, Germany. Phone: 49/251-83 55 361. Fax: 49/251-83 55 341. E-mail: hkarch{at}uni-muenster.de.

Applied and Environmental Microbiology, January 2005, p. 558-561, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.558-561.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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