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Applied and Environmental Microbiology, January 2005, p. 562-565, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.562-565.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Maturation by LctT Is Required for Biosynthesis of Full-Length Lantibiotic Lacticin 481

Patricia Uguen ,{dagger},{ddagger} Thomas Hindré,{dagger} Sandrine Didelot,§ Christel Marty, Dominique Haras, Jean-Paul Le Pennec, Karine Vallée-Réhel, and Alain Dufour*

Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne Sud, Lorient, France

Received 31 March 2004/ Accepted 29 August 2004

In lantibiotic lacticin 481 biosynthesis, LctT cleaves the precursor peptide and exports mature lantibiotic. Matrix-assisted laser desorption ionization-time of flight mass spectrometry revealed that a truncated form of lacticin 481 is produced in the absence of LctT or after cleavage site inactivation. Production of truncated lacticin 481 is 4-fold less efficient, and its specific activity is about 10-fold lower.

* Corresponding author. Mailing address: Laboratoire de Biotechnologie et Chimie Marines, Université de Bretagne Sud, BP 92116, 56321 Lorient Cedex, France. Phone: (33) 2-97-87-45-93. Fax: (33) 2-97-87-45-00. E-mail: alain.dufour{at}univ-ubs.fr.

{dagger} P. Uguen and T. Hindré contributed equally to this work.

{ddagger} Present address: Laboratoire Signalisation, Développement et Cancer, Université d'Orsay, 91405 Orsay, France.

§ Present address: Laboratoire de Biotechnologies et de Chimie Bio-Organique, Université de La Rochelle, 17042 La Rochelle, France.

Applied and Environmental Microbiology, January 2005, p. 562-565, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.562-565.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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