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Applied and Environmental Microbiology, January 2005, p. 569-573, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.569-573.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mutation of a LysR-Type Regulator of Antifungal Activity Results in a Growth Advantage in Stationary Phase Phenotype in Pseudomonas aureofaciens PA147-2

Mark W. Silby,1,2* Stephen R. Giddens,1,2,{dagger} and H. Khris Mahanty1

Department of Plant and Microbial Sciences,1 New Zealand Institute for Gene Ecology, University of Canterbury, Christchurch, New Zealand2

Received 6 May 2004/ Accepted 16 August 2004

The growth advantage in stationary phase (GASP) phenotype was shown to be present in two mutants lacking the antifungal phenotype (Af– mutants) of Pseudomonas aureofaciens PA147-2. Complementation demonstrated a correlation between GASP and the antifungal defect in one strain but not in the second. Sequence analysis revealed the Af– GASP strain had a mutation in a gene (finR) encoding a LysR-type regulator. Antifungal-minus mutants arose in starved cultures, and those aged cultures had increased fitness. Taken together, the results show that there are at least two paths to the GASP phenotype in P. aureofaciens, one of which results in a concomitant loss of the antifungal phenotype.

* Corresponding author. Present address: Center for Adaptation Genetics and Drug Resistance, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-2130. Fax: (617) 636-0458. E-mail: mark.silby{at}tufts.edu.

{dagger} Present address: Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.

Applied and Environmental Microbiology, January 2005, p. 569-573, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.569-573.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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