This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Valentine, N. Articles by Wahl, K. Search for Related Content PubMed PubMed Citation Articles by Valentine, N. Articles by Wahl, K. Agricola Articles by Valentine, N. Articles by Wahl, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2005, p. 58-64, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.58-64.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Effect of Culture Conditions on Microorganism Identification by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Pacific Northwest National Laboratory, Richland, Washington

Received 20 May 2004/ Accepted 23 August 2004

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identification with MALDI-TOF MS is still possible with the protocol established at the Pacific Northwest National Laboratory (K. H. Jarman, S. T. Cebula, A. J. Saenz, C. E. Petersen, N. B. Valentine, M. T. Kingsley, and K. L. Wahl, Anal. Chem. 72:1217-1223, 2000). A core set of small proteins remain constant under at least four different culture media conditions and blood agar plates, including minimal medium M9, rich media, tryptic soy broth (TSB) or Luria-Bertani (LB) broth, and blood agar plates, such that analysis of the intact cells by matrix-assisted laser desorption/ionization mass spectrometry allows for consistent identification.

This article has been cited by other articles:

• Jarman, K. H., Kreuzer-Martin, H. W., Wunschel, D. S., Valentine, N. B., Cliff, J. B., Petersen, C. E., Colburn, H. A., Wahl, K. L. (2008). Bayesian-Integrated Microbial Forensics. Appl. Environ. Microbiol. 74: 3573-3582 [Abstract] [Full Text]  
• Carbonnelle, E., Beretti, J.-L., Cottyn, S., Quesne, G., Berche, P., Nassif, X., Ferroni, A. (2007). Rapid Identification of Staphylococci Isolated in Clinical Microbiology Laboratories by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. J. Clin. Microbiol. 45: 2156-2161 [Abstract] [Full Text]  
• Kreuzer-Martin, H. W., Jarman, K. H. (2007). Stable Isotope Ratios and Forensic Analysis of Microorganisms. Appl. Environ. Microbiol. 73: 3896-3908 [Abstract] [Full Text]  
• Pignone, M., Greth, K. M., Cooper, J., Emerson, D., Tang, J. (2006). Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry.. J. Clin. Microbiol. 44: 1963-1970 [Abstract] [Full Text]  
• Mazzeo, M. F., Sorrentino, A., Gaita, M., Cacace, G., Di Stasio, M., Facchiano, A., Comi, G., Malorni, A., Siciliano, R. A. (2006). Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms. Appl. Environ. Microbiol. 72: 1180-1189 [Abstract] [Full Text]