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Applied and Environmental Microbiology, January 2005, p. 587-590, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.587-590.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of glnQ as a Counterselectable Marker for Creation of Allelic Exchange Mutations in Group B Streptococci

Glen S. Tamura,* Debra S. Bratt, Harry H. Yim, and Aphakorn Nittayajarn

Division of Infectious Disease, Department of Pediatrics, Children's Hospital and Regional Medical Center and University of Washington, Seattle, Washington

Received 30 March 2004/ Accepted 14 May 2004

Efficient allelic exchange mutagenesis in group B streptococci (GBS) has been hampered by the lack of a counterselectable marker system. Growth inhibition of GBS by the glutamine analog gamma-glutamyl hydrazide requires glnQ. We have used this phenomenon to create a counterselectable marker system for efficient selection of allelic exchange mutants in GBS.

* Corresponding author. Mailing address: Division of Infectious Disease and Department of Pediatrics, Children's Hospital and Regional Medical Center and University of Washington, 4800 Sand Point Way N.E., Seattle, WA 98105. Phone: (206) 987-2073. Fax: (206) 987-7311. E-mail: gtamura{at}u.washington.edu.

Applied and Environmental Microbiology, January 2005, p. 587-590, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.587-590.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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