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Applied and Environmental Microbiology, October 2005, p. 6049-6053, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6049-6053.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Rotavirus Virus-Like Particles as Surrogates To Evaluate Virus Persistence in Shellfish

Fabienne Loisy,1 Robert L. Atmar,2 Jean-Claude Le Saux,1 Jean Cohen,3 Marie-Paule Caprais,1 Monique Pommepuy,1 and Françoise S. Le Guyader1*

Laboratoire de Microbiologie, IFREMER, BP 21 105, 44311 Nantes cedex 03, France,1 Department of Molecular Virology and Microbiology, Baylor College of Medicine, 1 Baylor Plaza, Houston, Texas 77030,2 Virologie Moleculaire et Structurale, Unité mixte CNRS-INRA, 1 av. de la Terrasse, 91198 Gif-sur-Yvette, France3

Received 10 March 2005/ Accepted 25 May 2005

Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log10 of VLPs was depurated after 1 week in warm seawater (22°C). Phage MS2 was depurated more rapidly (about 2 log10 in 1 week) than were VLPs, as determined using a single-compartment model and linear regression analysis. After being relayed in the estuary under the influence of the tides, VLPs were detected in oysters for up to 82 days following seeding with high levels of VLPs (concentration range between 1010 and 109 particles per g of pancreatic tissue) and for 37 days for lower contamination levels (105 particles per g of pancreatic tissue). These data suggest that viral particles may persist in shellfish tissues for several weeks.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, IFREMER, BP 21 105, 44 311 Nantes cedex 03, France. Phone: 33 2 40 37 40 52. Fax: 33 2 40 37 40 73. E-mail: sleguyad{at}ifremer.fr.

Applied and Environmental Microbiology, October 2005, p. 6049-6053, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6049-6053.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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