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Applied and Environmental Microbiology, October 2005, p. 6115-6120, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6115-6120.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Lactobacillus acidophilus Strain of Human Gastrointestinal Microbiota Origin Elicits Killing of Enterovirulent Salmonella enterica Serovar Typhimurium by Triggering Lethal Bacterial Membrane Damage

Marie-Hélène Coconnier-Polter, Vanessa Liévin-Le Moal, and Alain L. Servin*

Institut National de la Santé et de la Recherche Médicale, Unité 510, Pathogènes et Fonctions des Cellules Epithéliales Polarisées, Faculté de Pharmacie, Université Paris XI, F-92296 Chatenay-Malabry, France

Received 15 March 2005/ Accepted 27 May 2005

The human gastrointestinal microbiota produces antagonistic activities against gastrointestinal bacterial pathogens. We undertook a study to investigate the mechanism(s) by which a Lactobacillus acidophilus strain of human microbiota origin antagonizes the gram-negative enteroinvasive pathogen Salmonella enterica serovar Typhimurium. We showed that the cell-free culture supernatant of L. acidophilus strain LB (LB-CFCS) induced the following effects in S. enterica SL1344: (i) a decrease in intracellular ATP that paralleled bacterial death, (ii) the release of lipopolysaccharide, (iii) permeabilization of the bacterial membrane, and (iv) an increase in the sensitivity of Salmonella to the lytic action of sodium dodecyl sulfate. Finally, we showed using two mutant strains of Salmonella, PhoP MS7953s and PmrA JKS1170, that the two-component regulatory systems PhoP-PhoQ and PmrA-PmrB that regulate the mechanisms of resistance to antibacterial agents in Salmonella did not influence the anti-Salmonella effect of LB-CFCS.

* Corresponding author. Mailing address: Faculté de Pharmacie, Unité Inserm 510, F-92296 Chatenay-Malabry, France. Phone: (33) 1 46 83 56 61. Fax: (33) 1 46 83 58 44. E-mail: alain.servin{at}cep.u-psud.fr.

Applied and Environmental Microbiology, October 2005, p. 6115-6120, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6115-6120.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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