This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ventura, M.
Right arrow Articles by van Sinderen, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ventura, M.
Right arrow Articles by van Sinderen, D.
Agricola
Right arrow Articles by Ventura, M.
Right arrow Articles by van Sinderen, D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2005, p. 6282-6291, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6282-6291.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genetic and Transcriptional Organization of the clpC Locus in Bifidobacterium breve UCC 2003

Marco Ventura,* Gerald F. Fitzgerald, and Douwe van Sinderen

Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland

Received 7 December 2004/ Accepted 13 April 2005

A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial species. Analysis by slot blot, Northern blot, and primer extension experiments showed that transcription of clpC is induced in response to moderate heat shock regimes. Moreover, we identified in the genome sequence of B. breve UCC 2003 a gene, designated clgR, which is predicted to encode a transcriptional regulator involved in regulation of the bifidobacterial clpC gene. The role of this protein in the regulation of B. breve UCC 2003 clpC gene expression was investigated by performing gel retardation experiments. We show that a biologically active ClgR molecule requires one or more proteinaceous coactivators to assist in the specific binding of ClgR to the clpC promoter region.

* Corresponding author. Mailing address: Department of Microbiology, National University of Ireland, Western Road, Cork, Ireland. Phone: 353-21-4901366. Fax: 353-21-4903031. E-mail: m.ventura{at}ucc.ie.

Applied and Environmental Microbiology, October 2005, p. 6282-6291, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6282-6291.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Turroni, F., Foroni, E., Pizzetti, P., Giubellini, V., Ribbera, A., Merusi, P., Cagnasso, P., Bizzarri, B., de'Angelis, G. L., Shanahan, F., van Sinderen, D., Ventura, M. (2009). Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract. Appl. Environ. Microbiol. 75: 1534-1545 [Abstract] [Full Text]  
  • Sheehan, V. M., Sleator, R. D., Hill, C., Fitzgerald, G. F. (2007). Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003. Microbiology 153: 3563-3571 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Zhang, Z., Fitzgerald, G. F., van Sinderen, D. (2007). Molecular Characterization of hsp20, Encoding a Small Heat Shock Protein of Bifidobacterium breve UCC2003. Appl. Environ. Microbiol. 73: 4695-4703 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Casale, A. D., Dellaglio, F., Neviani, E., Fitzgerald, G. F., van Sinderen, D. (2006). Analysis of bifidobacterial evolution using a multilocus approach. Int. J. Syst. Evol. Microbiol. 56: 2783-2792 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Bernini, V., Del Casale, A., Dellaglio, F., Neviani, E., Fitzgerald, G. F., van Sinderen, D. (2005). Genetic Characterization of the Bifidobacterium breve UCC 2003 hrcA Locus. Appl. Environ. Microbiol. 71: 8998-9007 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»