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Applied and Environmental Microbiology, October 2005, p. 6431-6433, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6431-6433.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Comparison of Rectoanal Mucosal Swab Cultures and Fecal Cultures for Determining Prevalence of Escherichia coli O157:H7 in Feedlot Cattle

M. A. Greenquist,¹ J. S. Drouillard,¹ J. M. Sargeant,^2, B. E. Depenbusch,¹ Xiaorong Shi,² K. F. Lechtenberg,³ and T. G. Nagaraja^2*

Department of Animal Sciences and Industry,¹ Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas 66506,² Midwest Veterinary Services, Inc., Oakland, Nebraska 68045³

Received 17 February 2005/ Accepted 24 May 2005

We compared fecal samples with samples collected with rectoanal mucosa swabs (RAMS) to determine the prevalence of Escherichia coli O157 in feedlot cattle (n = 747). Escherichia coli O157 was detected in 9.5% of samples collected with RAMS and 4.7% of samples tested by fecal culture. Pulsed-field gel electrophoresis analysis of isolates suggested that the strains colonizing the rectoanal junction were the same as those from the feces. Mucosal swab sampling was more sensitive than fecal sampling for determining the prevalence of E. coli O157 in feedlot cattle.

Present address: Department of Clinical Epidemiology and Biostatistics, Health Sciences Centre, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.

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