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Applied and Environmental Microbiology, October 2005, p. 6446-6452, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6446-6452.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Community Structure and Diversity of Biofilms from a Beer Bottling Plant as Revealed Using 16S rRNA Gene Clone Libraries{dagger}

Markus Timke, Ngoc Quynh Wang-Lieu, Karlheinz Altendorf, and André Lipski*

Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, 49069 Osnabrück, Germany

Received 6 December 2004/ Accepted 29 May 2005

The microbial composition of biofilms from a beer bottling plant was analyzed by a cultivation independent analysis of the 16S rRNA genes. Clone libraries were differentiated by amplified 16S rRNA gene restriction analysis and representative clones from each group were sequenced. The diversity of the clone libraries was comparable with the diversity found for environmental samples. No evidences for the presence of strictly anaerobic taxa or important beer spoilers were found, indicating that biofilms developed for more than 6 months at the plant formed no appropriate habitat for those microorganisms. The genus Methylobacterium was one of the dominating groups of the clone libraries. The size of this population was assessed by fluorescence in situ hybridization and fatty acid analysis. In addition, considerable numbers of clones were assigned to uncultivated organisms.

* Corresponding author. Mailing address: Universität Osnabrück, Fachbereich Biologie/Chemie, Abteilung Mikrobiologie, 49069 Osnabrück, Germany. Phone: (49) 541-969-2276. Fax: (49) 541-969-2870. E-mail: Lipski{at}Biologie.uni-osnabrueck.de.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, October 2005, p. 6446-6452, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6446-6452.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Herzog, P., Winkler, I., Wolking, D., Kampfer, P., Lipski, A. (2008). Chryseobacterium ureilyticum sp. nov., Chryseobacterium gambrini sp. nov., Chryseobacterium pallidum sp. nov. and Chryseobacterium molle sp. nov., isolated from beer-bottling plants. Int. J. Syst. Evol. Microbiol. 58: 26-33 [Abstract] [Full Text]  


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