This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, W.-T.
Right arrow Articles by Selamat, E. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, W.-T.
Right arrow Articles by Selamat, E. S.
Agricola
Right arrow Articles by Liu, W.-T.
Right arrow Articles by Selamat, E. S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2005, p. 6453-6457, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6453-6457.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Emission Characteristics of Fluorescent Labels with Respect to Temperature Changes and Subsequent Effects on DNA Microchip Studies

Wen-Tso Liu,* Jer-Horng Wu, Emily Sze-Ying Li, and Ezrein Shah Selamat

Department of Civil Engineering and Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576

Received 25 March 2005/ Accepted 31 May 2005

The effects of temperature, salt concentration, and formamide concentration on the emission characteristics of commonly used fluorescent labels were evaluated on DNA microchips. The emission intensities of different fluorophores without hybridization were observed to vary, each to a different extent, to mainly temperature changes. Rhodamine red, TAMRA (tetramethylrhodamine), and dyes from the carbocyanide group exhibited the largest variations, and Texas Red and Oregon Green exhibited the smallest variations. This temperature dependency was shown to affect results obtained during melting curve analysis in DNA microarray studies. To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed.

* Corresponding author. Mailing address: Department of Civil Engineering, National University of Singapore, Blk E1A, no. 07-03, Engineering Drive 2, Singapore 117576. Phone: 65-68741315. Fax: 65-67791635. E-mail: cveliuwt{at}nus.edu.sg.

Applied and Environmental Microbiology, October 2005, p. 6453-6457, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6453-6457.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Petersen, J., Poulsen, L., Petronis, S., Birgens, H., Dufva, M. (2008). Use of a multi-thermal washer for DNA microarrays simplifies probe design and gives robust genotyping assays. Nucleic Acids Res 36: e10-e10 [Abstract] [Full Text]  
  • Pozhitkov, A. E., Stedtfeld, R. D., Hashsham, S. A., Noble, P. A. (2007). Revision of the nonequilibrium thermal dissociation and stringent washing approaches for identification of mixed nucleic acid targets by microarrays. Nucleic Acids Res 35: e70-e70 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»