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Applied and Environmental Microbiology, October 2005, p. 6458-6462, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6458-6462.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Analysis of Methane Monooxygenase Genes in Mono Lake Suggests That Increased Methane Oxidation Activity May Correlate with a Change in Methanotroph Community Structure

Ju-Ling Lin,¹ Samantha B. Joye,² Johannes C. M. Scholten,¹ Hendrik Schäfer,¹ Ian R. McDonald,³ and J. Colin Murrell^1*

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England,¹ Department of Marine Sciences, University of Georgia, Athens, Georgia,² Department of Biological Sciences, University of Waikato, Hamilton, New Zealand³

Received 25 March 2005/ Accepted 19 May 2005

Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. Stratification of Mono Lake resulted in variation of aerobic methane oxidation rates with depth. Methanotroph diversity as determined by analysis of pmoA using new denaturing gradient gel electrophoresis primers suggested that variations in methane oxidation activity may correlate with changes in methanotroph community composition.

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* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England. Phone: 44 02476 523553. Fax: 44 02476 523568. E-mail: j.c.murrell{at}warwick.ac.uk.

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Applied and Environmental Microbiology, October 2005, p. 6458-6462, Vol. 71, No. 10
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.10.6458-6462.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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