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Applied and Environmental Microbiology, November 2005, p. 6664-6672, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6664-6672.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Impact of Different In Vitro Electron Donor/Acceptor Conditions on Potential Chemolithoautotrophic Communities from Marine Pelagic Redoxclines

Matthias Labrenz,1* Günter Jost,1 Christa Pohl,1 Sabrina Beckmann,1 Willm Martens-Habbena,2 and Klaus Jürgens1

Leibniz-Institut für Ostseeforschung Warnemünde, Seestrasse 15, 18119 Rostock-Warnemünde, Germany,1 Institut für Chemie und Biologie des Meeres, Universität Oldenburg, Carl-von-Ossietzky Strasse 9-11, 26111 Oldenburg, Germany2

Received 28 April 2005/ Accepted 21 June 2005

Anaerobic or microaerophilic chemolithoautotrophic bacteria have been considered to be responsible for CO2 dark fixation in different pelagic redoxclines worldwide, but their involvement in redox processes is still not fully resolved. We investigated the impact of 17 different electron donor/acceptor combinations in water of pelagic redoxclines from the central Baltic Sea on the stimulation of bacterial CO2 dark fixation as well as on the development of chemolithoautotrophic populations. In situ, the highest CO2 dark fixation rates, ranging from 0.7 to 1.4 µmol liter–1 day–1, were measured directly below the redoxcline. In enrichment experiments, chemolithoautotrophic CO2 dark fixation was maximally stimulated by the addition of thiosulfate, reaching values of up to 9.7 µmol liter–1 CO2 day–1. Chemolithoautotrophic nitrate reduction proved to be an important process, with rates of up to 33.5 µmol liter–1 NO3– day–1. Reduction of Fe(III) or Mn(IV) was not detected; nevertheless, the presence of these potential electron acceptors influenced the development of stimulated microbial assemblages. Potential chemolithoautotrophic bacteria in the enrichment experiments were displayed on 16S ribosomal complementary DNA single-strand-conformation polymorphism fingerprints and identified by sequencing of excised bands. Sequences were closely related to chemolithoautotrophic Thiomicrospira psychrophila and Maorithyas hadalis gill symbiont (both Gammaproteobacteria) and to an uncultured nitrate-reducing Helicobacteraceae bacterium (Epsilonproteobacteria). Our data indicate that this Helicobacteraceae bacterium could be of general importance or even a key organism for autotrophic nitrate reduction in pelagic redoxclines.

* Corresponding author. Mailing address: Leibniz-Institut für Ostseeforschung Warnemünde, Seestrasse 15, 18119 Rostock-Warnemünde, Germany. Phone: 49 (0)381 5197 378. Fax: 49 (0)381 5197 440. E-mail: matthias.labrenz{at}io-warnemuende.de.

Applied and Environmental Microbiology, November 2005, p. 6664-6672, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6664-6672.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kellermann, C., Griebler, C. (2009). Thiobacillus thiophilus sp. nov., a chemolithoautotrophic, thiosulfate-oxidizing bacterium isolated from contaminated aquifer sediments. Int. J. Syst. Evol. Microbiol. 59: 583-588 [Abstract] [Full Text]  
  • Grote, J., Jost, G., Labrenz, M., Herndl, G. J., Jurgens, K. (2008). Epsilonproteobacteria Represent the Major Portion of Chemoautotrophic Bacteria in Sulfidic Waters of Pelagic Redoxclines of the Baltic and Black Seas. Appl. Environ. Microbiol. 74: 7546-7551 [Abstract] [Full Text]  
  • Grote, J., Labrenz, M., Pfeiffer, B., Jost, G., Jurgens, K. (2007). Quantitative Distributions of Epsilonproteobacteria and a Sulfurimonas Subgroup in Pelagic Redoxclines of the Central Baltic Sea. Appl. Environ. Microbiol. 73: 7155-7161 [Abstract] [Full Text]  


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