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Applied and Environmental Microbiology, November 2005, p. 6673-6679, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6673-6679.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Bacteria Associated with Spores of the Arbuscular Mycorrhizal Fungi Glomus geosporum and Glomus constrictum

David Roesti,1* Kurt Ineichen,2 Olivier Braissant,3 Dirk Redecker,2 Andres Wiemken,2 and Michel Aragno1

Laboratory of Microbiology, University of Neuchâtel, Neuchâtel, Switzerland,1 Institute of Botany, University of Basel, Basel, Switzerland,2 Institute of Geology, University of Neuchâtel, Neuchâtel, Switzerland3

Received 6 December 2004/ Accepted 23 June 2005

Spores of the arbuscular mycorrhizal fungi (AMF) Glomus geosporum and Glomus constrictum were harvested from single-spore-derived pot cultures with either Plantago lanceolata or Hieracium pilosella as host plants. PCR-denaturing gradient gel electrophoresis analysis revealed that the bacterial communities associated with the spores depended more on AMF than host plant identity. The composition of the bacterial populations linked to the spores could be predominantly influenced by a specific spore wall composition or AMF exudate rather than by specific root exudates. The majority of the bacterial sequences that were common to both G. geosporum and G. constrictum spores were affiliated with taxonomic groups known to degrade biopolymers (Cellvibrio, Chondromyces, Flexibacter, Lysobacter, and Pseudomonas). Scanning electron microscopy of G. geosporum spores revealed that these bacteria are possibly feeding on the outer hyaline spore layer. The process of maturation and eventual germination of AMF spores might then benefit from the activity of the surface microorganisms degrading the outer hyaline wall layer.

* Corresponding author. Mailing address: Laboratory of Microbiology, University of Neuchâtel, CP2, CH-2007, Neuchâtel, Switzerland. Phone: (0041) 32 718.22.64. Fax: (0041) 32 718.22.31. E-mail: david.roesti{at}unine.ch.

Applied and Environmental Microbiology, November 2005, p. 6673-6679, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.6673-6679.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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