This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stepkowski, T.
Right arrow Articles by Howieson, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stepkowski, T.
Right arrow Articles by Howieson, J.
Agricola
Right arrow Articles by Stepkowski, T.
Right arrow Articles by Howieson, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2005, p. 7041-7052, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7041-7052.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

European Origin of Bradyrhizobium Populations Infecting Lupins and Serradella in Soils of Western Australia and South Africa{dagger} ,{ddagger}

Tomasz Stepkowski,1* Lionel Moulin,2 Agnieszka Krzyzanska,1 Alison McInnes,3 Ian J. Law,4 and John Howieson5

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland,1 Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD-CIRAD-INRA-AGROM, 34398 Montpellier cedex 5, France,2 School of Earth and Geographical Sciences, The University of Western Australia, 35 Stirling Hwy, Nedlands, WA 6009, Australia,3 Plant Protection Research Institute, Private Bag X134, Queenswood 0121, South Africa,4 Centre for Rhizobium Studies, Murdoch University, Murdoch, WA 6150, Australia5

Received 21 April 2005/ Accepted 19 July 2005

We applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin Bradyrhizobium strains that persist in soils of Western Australia and South Africa. The selected strains belonged to different randomly amplified polymorphic DNA (RAPD)-PCR clusters that were distinct from RAPD clusters of applied inoculant strains. Phylogenetic analyses were performed with nodulation genes (nodA, nodZ, nolL, noeI), housekeeping genes (dnaK, recA, glnII, atpD), and 16S-23S rRNA intergenic transcribed spacer sequences. Housekeeping gene phylogenies revealed that all serradella and Lupinus cosentinii isolates from Western Australia and three of five South African narrow-leaf lupin strains were intermingled with the strains of Bradyrhizobium canariense, forming a well supported branch on each of the trees. All nodA gene sequences of the lupin and serradella bradyrhizobia formed a single branch, referred to as clade II, together with the sequences of other lupin and serradella strains. Similar patterns were detected in nodZ and nolL trees. In contrast, nodA sequences of the strains isolated from native Australian legumes formed either a new branch called clade IV or belonged to clade I or III, whereas their nonsymbiotic genes grouped outside the B. canariense branch. These data suggest that the lupin and serradella strains, including the strains from uncultivated L. cosentinii plants, are descendants of strains that most likely were brought from Europe accidentally with lupin and serradella seeds. The observed dominance of B. canariense strains may be related to this species' adaptation to acid soils common in Western Australia and South Africa and, presumably, to their intrinsic ability to compete for nodulation of lupins and serradella.

* Corresponding author. Mailing address: Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61 704 Poznan, Noskowskiego 12/14, Poland. Phone: 48 61 852 85 03, ext. 149. Fax: 48 61 852 05 32. E-mail: sttommic{at}ibch.poznan.pl.

{dagger} We dedicate this work to Andrzej B. Legocki on the occasion of his 66th birthday.

{ddagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2005, p. 7041-7052, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7041-7052.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vinuesa, P., Rojas-Jimenez, K., Contreras-Moreira, B., Mahna, S. K., Prasad, B. N., Moe, H., Selvaraju, S. B., Thierfelder, H., Werner, D. (2008). Multilocus Sequence Analysis for Assessment of the Biogeography and Evolutionary Genetics of Four Bradyrhizobium Species That Nodulate Soybeans on the Asiatic Continent. Appl. Environ. Microbiol. 74: 6987-6996 [Abstract] [Full Text]  
  • Andam, C. P., Parker, M. A. (2007). Novel Alphaproteobacterial Root Nodule Symbiont Associated with Lupinus texensis. Appl. Environ. Microbiol. 73: 5687-5691 [Abstract] [Full Text]  
  • Rodriguez-Echeverria, S., Crisostomo, J. A., Freitas, H. (2007). Genetic Diversity of Rhizobia Associated with Acacia longifolia in Two Stages of Invasion of Coastal Sand Dunes. Appl. Environ. Microbiol. 73: 5066-5070 [Abstract] [Full Text]  
  • Stepkowski, T., Hughes, C. E., Law, I. J., Markiewicz, L., Gurda, D., Chlebicka, A., Moulin, L. (2007). Diversification of Lupine Bradyrhizobium Strains: Evidence from Nodulation Gene Trees. Appl. Environ. Microbiol. 73: 3254-3264 [Abstract] [Full Text]  
  • Whitaker, R. J (2006). Allopatric origins of microbial species. Phil Trans R Soc B 361: 1975-1984 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»