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Applied and Environmental Microbiology, November 2005, p. 7217-7223, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7217-7223.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

16S rRNA Gene-Based Identification of Midgut Bacteria from Field-Caught Anopheles gambiae Sensu Lato and A. funestus Mosquitoes Reveals New Species Related to Known Insect Symbionts

Jenny M. Lindh, Olle Terenius, and Ingrid Faye*

Department of Genetics, Microbiology and Toxicology, Stockholm University, 106 91 Stockholm, Sweden

Received 18 January 2005/ Accepted 10 July 2005

Field-collected mosquitoes of the two main malaria vectors in Africa, Anopheles gambiae sensu lato and Anopheles funestus, were screened for their midgut bacterial contents. The midgut from each blood-fed mosquito was screened with two different detection pathways, one culture independent and one culture dependent. Bacterial species determination was achieved by sequence analysis of 16S rRNA genes. Altogether, 16 species from 14 genera were identified, 8 by each method. Interestingly, several of the bacteria identified are related to bacteria known to be symbionts in other insects. One isolate, Nocardia corynebacterioides, is a relative of the symbiont found in the vector for Chagas' disease that has been proven useful as a paratransgenic bacterium. Another isolate is a novel species within the {gamma}-proteobacteria that could not be phylogenetically placed within any of the known orders in the class but is close to a group of insect symbionts. Bacteria representing three intracellular genera were identified, among them the first identifications of Anaplasma species from mosquitoes and a new mosquito-Spiroplasma association. The isolates will be further investigated for their suitability for a paratransgenic Anopheles mosquito.

* Corresponding author. Mailing address: Department of Genetics, Microbiology and Toxicology, Stockholm University, 106 91 Stockholm, Sweden. Phone: 46 (0)8 161272. Fax: 46 (0)8 164315. E-mail: Ingrid.Faye{at}gmt.su.se.

Applied and Environmental Microbiology, November 2005, p. 7217-7223, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7217-7223.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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