AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lehours, A.-C.
Right arrow Articles by Fonty, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lehours, A.-C.
Right arrow Articles by Fonty, G.
Agricola
Right arrow Articles by Lehours, A.-C.
Right arrow Articles by Fonty, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2005, p. 7389-7400, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7389-7400.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Anaerobic Microbial Communities in Lake Pavin, a Unique Meromictic Lake in France{dagger}

Anne-C. Lehours,* Corinne Bardot, Aurelie Thenot, Didier Debroas, and Gerard Fonty

Laboratoire de Biologie des Protistes, UMR CNRS 6023, Université Blaise Pascal, 63177 Aubière Cedex, France

Received 6 January 2005/ Accepted 13 July 2005

The Bacteria and Archaea from the meromictic Lake Pavin were analyzed in samples collected along a vertical profile in the anoxic monimolimnion and were compared to those in samples from the oxic mixolimnion. Nine targeted 16S rRNA oligonucleotide probes were used to assess the distribution of Bacteria and Archaea and to investigate the in situ occurrence of sulfate-reducing bacteria and methane-producing Archaea involved in the terminal steps of the anaerobic degradation of organic material. The diversity of the complex microbial communities was assessed from the 16S rRNA polymorphisms present in terminal restriction fragment (TRF) depth patterns. The densities of the microbial community increased in the anoxic layer, and Archaea detected with probe ARCH915 represented the largest microbial group in the water column, with a mean Archaea/Eubacteria ratio of 1.5. Terminal restriction fragment length polymorphism (TRFLP) analysis revealed an elevated archaeal and bacterial phylotype richness in anoxic bottom-water samples. The structure of the Archaea community remained rather homogeneous, while TRFLP patterns for the eubacterial community revealed a heterogeneous distribution of eubacterial TRFs.

* Corresponding author. Mailing address: Laboratoire de Biologie des Protistes, UMR CNRS 6023, 24 avenue des Landais, 63177 Aubière Cedex, France. Phone: (33)8473407751. Fax: (33)8473407670. E-mail: A-Catherine.LEHOURS{at}univ-bpclermont.fr.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, November 2005, p. 7389-7400, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7389-7400.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2005 by the American Society for Microbiology. All rights reserved.