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Applied and Environmental Microbiology, November 2005, p. 7515-7522, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7515-7522.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Isolation, Sequence Analysis, and Comparison of Two Plasmids (28 and 29 Kilobases) from the Biomining Bacterium Leptospirillum ferrooxidans ATCC 49879

Nicolette J. Coram, Leonardo J. van Zyl, and Douglas E. Rawlings*

Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa

Received 9 March 2005/ Accepted 28 June 2005

Two plasmids, of 28,878 bp and 28,012 bp, were isolated from Leptospirillum ferrooxidans ATCC 49879. Altogether, a total of 67 open reading frames (ORFs) were identified on both plasmids, of which 32 had predicted products with high homology to proteins of known function, while 11 ORFs had predicted products with homology to previously identified proteins of unknown function. Twenty-four ORFs had products with no homologues in the GenBank/NCBI database. An analysis of the ORFs and other features of the two plasmids, the first to be isolated from a bacterium of the genus Leptospirillum, is presented.

* Corresponding author. Mailing address: Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa. Phone: 27-21-808 5848. Fax: 27-21-808 5846. E-mail: der{at}sun.ac.za.

Applied and Environmental Microbiology, November 2005, p. 7515-7522, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7515-7522.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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