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Applied and Environmental Microbiology, November 2005, p. 7578-7581, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7578-7581.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Representational Difference Analysis and Real-Time PCR for Strain-Specific Quantification of Lactobacillus sobrius sp. nov.

Sergey R. Konstantinov,* Hauke Smidt, and Willem M. de Vos

Laboratory of Microbiology, Agrotechnology and Food Sciences Group, Wageningen University, Wageningen, The Netherlands

Received 14 April 2005/ Accepted 7 July 2005

Lactobacillus sobrius sp. nov., which was recently isolated from the intestine of weaning piglets, has potential probiotic properties. To follow the fate of L. sobrius strain 001T in dietary interventions, a novel and strain-specific quantitative detection procedure was developed. This procedure was based on the isolation of specific genomic fragments from the type strain by representational difference analysis and their detection by real-time PCR. The described strain-specific quantification approach may be used in studies aimed at tracking bacterial strains added to specific environments.

* Corresponding author. Mailing address: Laboratory of Microbiology, Agrotechnology and Food Sciences Group, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Phone: 31 317 483118. Fax: 31 317 483829. E-mail: sergey.konstantinov{at}wur.nl.

Applied and Environmental Microbiology, November 2005, p. 7578-7581, Vol. 71, No. 11
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.11.7578-7581.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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